anti human micb af750 (R&D Systems)

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R&D Systems anti human micb af750
Anti Human Micb Af750, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+micb/Mouse+CD6+Antibody/pm40816946-255-20-26
Average 92 stars, based on 33 article reviews

anti human micb af750 - by Bioz Stars, 2026-09

92/100 stars

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Bioprocessing:

Article Title: Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition
Article Snippet: .. The following monoclonal antibodies (mAbs) were used for immunostaining or as blocking Abs: anti-MICA (MAB1300), anti-MICB (MAB1599), anti-ULBP-1 (MAB1380), anti-ULBP-2/5/6 (MAB1298), anti-ULBP-3 (MAB1517), and anti-NKG2D (MAB139) were purchased from R&D System (Minneapolis, MN, USA). .. Anti-MHC class I (W6/32) was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

Article Title: Doxorubicin-Mediated miR-433 Expression on Exosomes Promotes Bystander Senescence in Multiple Myeloma Cells in a DDR-Independent Manner.
Article Snippet: For flow the cytometric determination of cellular senescence, SA-β-Gal activity was evaluated using the substrate C12FDG (Invitrogen), as reported in the literature [38]. .. Unconjugated monoclonal antibodies used for immunostaining were the following: anti-MICA (MAB159227) and anti-MICB (MAB236511) from R&D Systems (Minneapolis, MN, USA) and anti-PVR (46.31 or SKII.4), which was kindly provided by Prof. Colonna (Washington University in St. Louis, MO, USA). .. Allophycocyanin (APC)-conjugated goat affinity purified F(ab′)2 fragment to mouse IgG (GAM) was purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).

Immunostaining:

Blocking Assay:

Article Title: Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition
Article Snippet: .. Cells were stained with anti-MICB (MAB1599—R&D System) for 1 h followed by Alexa Fluor 488-conjugated goat antimouse IgG (A11001—Invitrogen, Life Technologies) for 1 h, all diluted in blocking buffer (PBS 0.01% Triton-X-100, 1% FBS). .. After extensive washing coverslips were mounted using SlowFade Gold Antifade Mountant with DAPI (S36938—Thermo Fisher Scientific).

Flow Cytometry:

Article Title: The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing
Article Snippet: .. The following primary antibodies were used for flow cytometry: anti-MICA (clone 159227; R&D Systems), anti-MICB (clone 236511, R&D Systems), anti-ULBP1 (clone 170818; R&D Systems), anti-ULBP2/5/6 (clone 165903; R&D Systems) and anti-ULBP3 (clone 166514, R&D Systems). .. The following primary antibodies were used for immunofluorescence: anti-PDI (ab3672, Abcam), anti-FLAG tag (Clone L5, Biolegend) and anti-MICA (clone 159227, R&D Systems).

Article Title: Reovirus infection of tumor cells reduces the expression of NKG2D ligands, leading to impaired NK-cell cytotoxicity and functionality.
Article Snippet: .. The following primary antibodies were used for flow cytometry: anti-MICA (R&D, MAB1300), anti-MICB (R&D, MAB1599), antiULBP1 (R&D, MAB1380), anti-ULBP2 (R&D, MAB1298, with cross reactivity to ULBP5 and ULBP6), anti-ULBP3 (R&D, MAB1517), anti-GLUT-1 (abcam, Ab150299), anti-IFNƔ (R&D, MAB285) , ant i -CD56 (BioLegend, 36503) , ant i -CD3 (ThermoFisher Scientific, 56-0038-82), anti-s1 (Sigma-Aldrich, MAB994-I-25UG), anti-CD4 (BioLegend, 344604), anti-CRT (Invitrogen, 902A), anti-ERp57 (BioLegend, 937301) and antiPVR (BioLegend, 337602). ..

Article Title: Reovirus infection of tumor cells reduces the expression of NKG2D ligands, leading to impaired NK-cell cytotoxicity and functionality
Article Snippet: .. The following primary antibodies were used for flow cytometry: anti-MICA (R&D, MAB1300), anti-MICB (R&D, MAB1599), anti-ULBP1 (R&D, MAB1380), anti-ULBP2 (R&D, MAB1298, with cross reactivity to ULBP5 and ULBP6), anti-ULBP3 (R&D, MAB1517), anti-GLUT-1 (abcam, Ab150299), anti-IFNƔ (R&D, MAB285), anti-CD56 (BioLegend, 36503), anti-CD3 (ThermoFisher Scientific, 56-0038-82), anti-σ1 (Sigma-Aldrich, MAB994-I-25UG), anti-CD4 (BioLegend, 344604), anti-CRT (Invitrogen, 902A), anti-ERp57 (BioLegend, 937301) and anti-PVR (BioLegend, 337602). ..

Staining:

Article Title: Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition
Article Snippet: .. Cells were stained with anti-MICB (MAB1599—R&D System) for 1 h followed by Alexa Fluor 488-conjugated goat antimouse IgG (A11001—Invitrogen, Life Technologies) for 1 h, all diluted in blocking buffer (PBS 0.01% Triton-X-100, 1% FBS). .. After extensive washing coverslips were mounted using SlowFade Gold Antifade Mountant with DAPI (S36938—Thermo Fisher Scientific).

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